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Inhibition of Macrophage Migration by Virus‐Induced Interferon Preparations 1
Author(s) -
Imanishi Jiro
Publication year - 1975
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1975.tb00889.x
Subject(s) - sephadex , interferon , newcastle disease , size exclusion chromatography , chemistry , virus , trypsin , macrophage migration inhibitory factor , in vitro , microbiology and biotechnology , inhibitory postsynaptic potential , molecular mass , biology , virology , biochemistry , immunology , cytokine , enzyme , endocrinology
It was found that a preparation of mouse L cell interferon induced by Newcastle disease virus (NDV) possessed not only interferon activity but also inhibitory activity upon migration of guinea pig peritoneal macrophages (MIF activity). These activities were also observed in a preparation of human leukocyte interferon induced by NDV. The interferon and MIF activities shared common characteristics in the dose response, time course of in vitro production, thermal stability, sensitivity to trypsin and periodate, and elution pattern in CM‐Sephadex column chromatography. However, gel filtration pattern with Sephadex G‐100 showed two separate peaks. Fractions collected from the first peak, corresponding to a molecular weight of about 45 000, had only the MIF activity, while those collected from the second peak, corresponding to a molecular weight of about 30 000, had both the interferon and MIF activities. A preparation of mouse brain interferon induced by Japanese encephalitis virus had a much weaker MIF activity than the L cell interferon, although these preparations were equal in interferon activity (5000 units/ml).