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Neutralizing Activities of Early and Late IgG Fragments from Rabbits Immunized with Herpes Simplex Virus
Author(s) -
Shinkai Kenkichi,
Yoshino Kamcsaburo
Publication year - 1975
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1975.tb00870.x
Subject(s) - neutralization , chemistry , herpes simplex virus , bivalent (engine) , antibody , virus , virology , immunoglobulin g , immunoglobulin fab fragments , herpes virus , microbiology and biotechnology , ultraviolet irradiation , biology , irradiation , immunology , immunoglobulin light chain , organic chemistry , metal , complementarity determining region , physics , nuclear physics
Rabbits immunized with herpes virus were bled periodically and bivalent and univalent fragments of IgG from each serum sample were prepared by enzymatic digestion. The 2‐week F(ab′) 2 showed a low neutralizing activity only after addition of anti‐IgG. F(ab′) 2 of the 4‐week serum retained almost all of the neutralizing activity of IgG, while its univalent fragments demonstrated none even when tested with anti‐IgG. In contrast to these early IgG fragments, univalent fragments of the 9‐week and 20‐week IgG neutralized the virus to considerable extents in the absence of anti‐IgG; after addition of anti‐IgG the activity equaled that of intact IgG in the cases of Fab′ and Fab‐II, though the activity of Fab‐I was relatively low. The three univalent fragments were all sensitive to heating at 70 C and to ultraviolet irradiation, whereas intact IgG resisted these treatments. F(ab′) 2 was resistant to the heating and less sensitive to ultraviolet irradiation than univalent fragments. Neutralization kinetic curve experiments to test blocking effects of IgG fragments against the neutralization by intact IgG suggested that the early Fab′ did combine with the virus and that the late Fab′ exerted a higher blocking effect than the early Fab′.