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The Skin‐Reactive Antigens of Mycoplasma pneumoniae
Author(s) -
Mizutani Hiroko,
Mizutani Hiromichi
Publication year - 1975
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1975.tb00862.x
Subject(s) - antigen , chloroform , titer , acetone , chemistry , mycoplasma pneumoniae , microbiology and biotechnology , mycoplasma , antibody , biology , chromatography , immunology , biochemistry , medicine , pneumonia
ABSTRACT Guinea pigs experimentally infected with Mycoplasma pneumoniae or immunized with the organism in combination with Freund's complete adjuvant developed a delayed hypersensitive skin reaction following on intradermal injection of the M. pneumoniae antigen. The amount of protein necessary to produce the delayed skin reaction was as low as 0.01 μg. When the sonicated whole cells were extracted with aqueous acetone, the delayed skin reactivity was found mostly in the acetone insoluble (lipid‐depleted) fraction. On the other hand, the lipid fraction which was isolated by a chloroform–methanol extraction of the acetone‐soluble fraction and had a high titer of complement‐fixing activity, exhibited little delayed skin reactivity. The lipid‐depleted antigens as the whole cell antigens produced delayed skin reactivities in human patients.