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Inhibiting Materials for γ Phage Adsorption to the Cell Wall of Bacillus anthracis , Strain Pasteur No. 2‐H
Author(s) -
Watanabe Takashi,
Shiomi Toshiro
Publication year - 1975
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1975.tb00857.x
Subject(s) - cell wall , bacillus anthracis , diaminopimelic acid , chemistry , strain (injury) , glucosamine , chromatography , alanine , hydrolysis , adsorption , biochemistry , microbiology and biotechnology , amino acid , peptidoglycan , bacteria , biology , organic chemistry , genetics , anatomy
Cell wall preparations of Bacillus anthracis , strain Pasteur No. 2‐H, were treated with heat or with acetone and ether. Both of the treated cell walls preparations inactivated γ phage. The centrifuged supernatant of the heat‐treated cell walls was fractionated on Sephadex G‐200, and four fractions containing reducing sugars were obtained. The first fraction had the phage‐inactivating activity. On the other hand, the fourth fraction had no phage‐inactivating activity, but strongly inhibited phage adsorption to the cell walls. In the fourth fraction, glutamic acid, alanine, 2, 6‐diaminopimelic acid and glucosamine were detected by paper chromatography after acid hydrolysis. Authentic D,L ‐2, 6‐diaminopimelic acid and D ‐glucosamine markedly inhibited phage adsorption to the cell walls. D ‐Galactosamine, D ‐mannosamine and L ‐lysine also showed similar activities. Results suggest the possibility that one or a combination of these substances defines the characteristics of phage adsorption to the cell walls of B. anthracis , strain Pasteur No. 2‐H.