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The Metabolic and Phagocytic Activities of Leukocytes from Patients Receiving Corticosteroid and Radiation Therapy, and Patients with Bacterial Infections
Author(s) -
Okuda Kenji,
Tadokoro Ichiro,
Noguchi Yoshikuni
Publication year - 1975
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1975.tb00856.x
Subject(s) - pentose phosphate pathway , nicotinamide adenine dinucleotide phosphate , dehydrogenase , nadph oxidase , enzyme , hydrogen peroxide , glucose 6 phosphate dehydrogenase , corticosteroid , medicine , oxidase test , microbiology and biotechnology , immunology , endocrinology , biology , biochemistry , pharmacology , glycolysis
Peripheral blood leukocytes from patients given corticosteroid or radiation therapy, as well as patients with bacterial or viral infections, were studied with regard to the selected enzyme activities of the hexose monophosphate shunt (HMS). Glucose‐6‐phosphate dehydrogenase (G‐6‐PD), 6‐phosphogluconate dehydrogenase (6‐PGD) and reduced nicotinamide‐adenine dinucleotide phosphate (NADPH) oxidase were assayed spectrophotometrically on mixed leukocyte suspensions in isotonic glycerol. Enzyme activities of G‐6‐PD and NADPH oxidase in patients receiving corticosteroid or radiation therapy were significantly lower than the enzyme activity of 6‐PGD. In patients with bacterial infections, activities of the three enzymes increased but in patients with viral infections, only the activities of NADPH oxidase and G‐6‐PD were slightly decreased. Nitroblue tetrazolium (NBT) dye‐reducing activities of neutrophils from patients receiving corticosteroid or radiation therapy were attenuated which coincides with the reduced activities of HMS enzymes. From these results, it is likely that the reduced activities of intraleukocytic HMS enzymes of patients receiving corticosteroid or radiation therapy are correlated with intracellular bactericidal activities which might result from the attenuated level of hydrogen peroxide production.