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Virucidal Effect of Sodium p ‐Chloromercuribenzoate on Influenza Viruses Attributable to Inhibition of Virus Particle‐Associated RNA‐Dependent RNA Polymerase
Author(s) -
Shinkai Kenkichi
Publication year - 1975
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1975.tb00843.x
Subject(s) - virus , rna polymerase , infectivity , virology , neuraminidase , rna , polymerase , incubation , chemistry , strain (injury) , influenza a virus , orthomyxoviridae , microbiology and biotechnology , biology , enzyme , biochemistry , gene , anatomy
Sodium p ‐chloromercuribenzoate (PCMB) caused a noticeable reduction of infectivity of prototype strains of type A and Lee strain of type B influenza viruses at concentrations of 100 and 200 μg/ml, respectively, after an incubation at 37 C for 60 min. The virucidal effect on A/AA/2/60 (H 2 N 2 ) strain was dependent on the concentration of the drug and temperature as well as on the time of incubation. The reagent exerted this effect at a concentration which induced little change in the hemagglutinating and neuraminidase activities of the virus. PCMB inhibited by 50% the virus particle‐associated RNA polymerase activity of all prototype strains of type A influenza virus at about 2 μg/ml and that of Lee strain of type B influenza virus at 8.5 μg/ml. Other sulfhydryl reagent such as phenylmercuric nitrate also exhibited virucidal effect on A/AA/2/60 virus which paralleled their inhibition of the virus particle‐associated RNA polymerase activity. From these results it was considered likely that the virucidal action of PCMB on influenza viruses was attributable to inhibition of the virus particle‐associated RNA polymerase activity.