Premium
Genetic and Biochemical Studies on Drug‐Resistant Mutants in Mycobacterium smegmatis
Author(s) -
Mizuguchi Yasuo,
Suga Kiyoko,
Masuda Kunitsugu,
Yamada Takeshi
Publication year - 1974
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1974.tb00834.x
Subject(s) - capreomycin , viomycin , mycobacterium smegmatis , kanamycin , mutant , neomycin , biology , streptomycin , complementation , microbiology and biotechnology , genetics , antibiotics , mycobacterium tuberculosis , gene , ethambutol , medicine , tuberculosis , pathology
Genetic analyses of the loci conferring resistance to antibiotics known to affect protein synthesis were made employing the conjugation system of Mycobacterium smegmatis . By the complementation tests, vic (viomycin–capreomycin‐resistant) mutants were classified into two different groups; cistrons A and B. Neomycin–kanamycin‐resistant ( nek ) mutants, on the contrary, fall into a single cistron. Erythromycin‐resistance ( ery ) locus was linked to vic–nek region. The map order deduced was argB, argA , ( vicA, vicB ), nek, ery . Biochemical studies showed that strains resistant to streptomycin, neomycin–kanamycin or viomycin–capreomycin had altered ribosomes.