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Production of Mouse Interferon with High Titers in a Large‐Scale Suspension Culture System 1
Author(s) -
Sano Emiko,
Matsui Yoshiko,
Kobayashi Sigeyasu
Publication year - 1974
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1974.tb00805.x
Subject(s) - newcastle disease , titer , suspension culture , suspension (topology) , cell culture , virology , interferon , virus , microbiology and biotechnology , chemistry , biology , homotopy , mathematics , pure mathematics , genetics
L‐MS cells, adapted to grow in suspension, were obtained by selection from a high interferon (IF)‐producing line of mouse L cell monolayers. A large volume of L‐MS cells (20 liters or more; 1–2 × 10 10 cells) was readily grown in a spinner culture, retaining their ability to produce high yields of IF in serum‐free medium following induction with Newcastle disease virus (NDV). The optimal condition for the production of IF in the suspension culture of L‐MS cells was established. The system also proved itself to be susceptible to IF induction by polyinosinic‐polycytidylic acid (Poly I · Poly C) and by NDV inactivated with ultraviolet light (NDV‐UV). By employing the present system, large quantities of mouse IF of a high titer could be routinely prepared.