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Transformability of Group H Streptococcus Challis
Author(s) -
Ito Takako,
Hirano Toyo,
Tomura Tsuneko,
Yoshioka Morimasa
Publication year - 1973
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1973.tb00929.x
Subject(s) - dna , hemolysis , streptococcus , chemistry , microbiology and biotechnology , genetics , biology , bacteria , biochemistry , immunology
Two types of hemolysis, α and β were formed by group H streptococcus wild type strain Challis on horse blood agar, and both of the hemolytic markers could be transformed in each. α‐Hemolysis‐acquired transformants lost the competence‐provoking factor producibility (CPF – ). When DNAs, obtained from strains Challis α, dihydrostreptomycin sulfate‐resistant Challis α, and Wicky, were used CPF – transformants appeared in high frequencies. DNAs from incompetent β cells grown in ET3 medium for 6 hr or in heated ET3 medium for 2 hr also transferred the CPF – . CPF producibility (CPF + ) was transformed with DNA from competent Challis β, while it was not transformed to Challis β with DNA from Wicky cells in competent and incompetent phases. Among β transformants obtained there were (1) those having CPF + and competency, (2) those having competency but CPF – , and also (3) those defective in both. Based upon these results, the transformability is discussed regarding close relation to DNA receptor site or CPF attachment and also on the probable transfer of itself by transforming DNA.