Premium
Intracellular Components Associated with Chikungunya Virus‐Specific Ribonucleic Acids in Infected BHK21 Cells 1
Author(s) -
Igarashi Akira,
Sasao Fuyoko,
Fukai Konosuke
Publication year - 1973
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1973.tb00791.x
Subject(s) - uridine , chemistry , ethylenediaminetetraacetic acid , puromycin , rna , intracellular , ultracentrifuge , sucrose , virus , cytoplasm , glutaraldehyde , virology , density gradient , nucleoprotein , biochemistry , microbiology and biotechnology , protein biosynthesis , biology , chromatography , chelation , physics , organic chemistry , quantum mechanics , gene
Cytoplasmic extracts of BHK21 cells infected with Chikungunya virus were analyzed by sucrose gradient sedimentation. Besides 140 S nucleocapsid, 65 S component associated with 26 S single‐stranded ribonucleic acid was labeled with 3 H‐uridine in actinomycin‐treated infected cells. The 65 S component, demonstrable in ethylenediaminetetraacetic acid‐containing buffers, appeared to accumulate when protein synthesis was inhibited. After glutaraldehyde fixation, this component had a density of 1.48–1.50 g/ml in CsCl. Pulse‐chase experiments did not indicate that the 65 S component was a precursor of the 140 S nucleocapsid.