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Detection of a Specific Surface Antigen(s) in Adenovirus Type 12‐Transformed Cells by Fluorescent Antibody Technique
Author(s) -
Hamada Chuya,
Nakajima Setsuko,
Uetake Hisao
Publication year - 1973
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1973.tb00776.x
Subject(s) - antigen , antiserum , antibody , microbiology and biotechnology , biology , virology , virus , pan t antigens , cell , immunology , monoclonal antibody , biochemistry
A surface antigen(s) was detected in adenovirus type 12 (Ad12)‐transformed cells by fluorescent antibody technique. The antigen was revealed by antisera of mice and rabbits immunized with the virus, and by those of rabbits immunized with conditioned medium from cell cultures carrying the surface antigen. The antigen was specific for the Ad12‐transformed cells, since it was found only in the Ad12‐transformed cells regardless of the animal species and not in any other cells, including SV40‐ and polyoma virus‐transformed cells. The antigen was distinct from the Ad12‐specific viral and T antigens, since the sera of rabbits immunized with the conditioned medium contained little or no detectable antibodies to the latter two antigens. On the contrary, the surface antigen was not detected in any tumor cells induced by Ad12 by the above antisera, and no detectable antibody to the surface antigen was produced in mice immunized with the tumor cells.