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Formation of the Recombinant between Ampicillin Re istance Determinant and Transfer Factor
Author(s) -
Nakajima Takashi,
Suzuki Mitsue,
Kameda Mitsuo,
Harada Kenji,
Mitsuhashi Susumu
Publication year - 1973
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1973.tb00770.x
Subject(s) - recombinant dna , ampicillin , microbiology and biotechnology , biology , amp resistance , enterobacter cloacae , mutant , chemistry , escherichia coli , enterobacteriaceae , antibiotics , biochemistry , gene
In vitro ‐developed resistant mutants were obtained by inoculating a clinical isolate of Aerobacter cloacae on plates containing various concentrations of ampicillin (APC). This resistance was paralleled by an increase in the formation of β‐lactamase. When A. cloacae carrying the nontransmissible APC resistance‐determinant ( amp ) was infected with a T‐ tet factor, a recombinant T‐ tet.amp factor was formed; the recombinant being conjugally transmissible and capable of conferring APC resistance on their host. This fact implies the origin of the R factor; the R factor being formed by the recombination of sex factors with nontransmissible drug resistance‐determinants.