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Studies on Temperate Phages of Lactobacillus salivarius
Author(s) -
Tohyama Kiyoshi
Publication year - 1973
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1973.tb00723.x
Subject(s) - lysogenic cycle , chloramphenicol , microbiology and biotechnology , reversion , kinetics , bacteriophage , lysis , temperateness , strain (injury) , biology , lactobacillus casei , chemistry , bacteria , biochemistry , antibiotics , escherichia coli , genetics , physics , quantum mechanics , anatomy , gene , phenotype
Comparative studies were carried out on the mode of action of the defective temperate phage 208 against the homologous lysogenic strain S‐208 and a nonlysogenic strain S‐161 of Lactobacillus salivarius . Treatment of both strains with phage 208 led to a specific inhibition of protein synthesis, cell killing without any reversion of protein synthesis, and of viable counts by treatment with trypsin. Killing action of phage 208 followed a single hit kinetics for nonlysogenic S‐161 and S‐208 No. 006, which is a cured strain of S‐208, whereas, two to five hit kinetics was obtained for lysogenic S‐208. Phage particles exposed to ultraviolet light (5.7 kergs/cm 2 ) also killed S‐161 with a single hit kinetics and S‐208 with a 3‐hit kinetics. However, the kinetics of killing approached a single hit when the protein synthesis of S‐208 was inhibited by chloramphenicol prior to the phage addition. Based upon these results, the possible mechanisms of immunity breakdown and of subsequent cell killing were discussed.