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The Possible Presence of Ribonucleic Acid‐Dependent Deoxyribonucleic Acid Polymerase in the Immune Response
Author(s) -
Kurashige Satonori,
Mitsuhashi Susumu
Publication year - 1973
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1973.tb00713.x
Subject(s) - rna , deoxyribonuclease , ribonuclease , microbiology and biotechnology , dna , polynucleotide , rna dependent rna polymerase , rna polymerase , enzyme , polymerase , biology , biochemistry , dna synthesis , chemistry , gene
Immune ribonucleic acid ( i RNA) preparations were extracted from spleens of rabbits immunized either with Salmonella flagella or f2 phage. The enzyme extract prepared from popliteal lymph node cells of immunized rabbits induced DNA synthesis in a cell‐free system in the presence of i RNA. The enzyme activity was demonstrated only after stimulation with a corresponding antigen and was inhibited by large amounts of rifampicin, rifampicin derivatives or by treatment of the i RNA with ribonuclease, but neither actinomycin D nor mitomycin C affected it. Pretreatment of i RNA with deoxyribonuclease did not affect the enzyme activity. This DNA polymerase could induce DNA synthesis only by using the corresponding i RNA but not by using either normal RNA or i RNA prepared against another antigen. This new DNA presumably represents an intermediate in the antibody formation process specified by this enzyme using i RNA as a template.