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Staphylococcal Enterotoxin D
Author(s) -
Igarashi Hideo
Publication year - 1972
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1972.tb00688.x
Subject(s) - chromatography , sephadex , enterotoxin , chemistry , precipitin , size exclusion chromatography , immunoelectrophoresis , ouchterlony double immunodiffusion , toxin , immunodiffusion , antiserum , staphylococcus aureus , polyethylene glycol , microbiology and biotechnology , biochemistry , antigen , biology , escherichia coli , bacteria , genetics , gene , enzyme
Purification of enterotoxin D from Staphylococcus aureus 494 was attempted by utilizing the following techniques: Concentration of bacterial culture supernatant with polyethylene glycol 20000, CM‐cellulose column chromatography, Sephadex G‐100 gel‐filtration, chromatography on a DEAE cellulose column with concave gradient elution and gel‐filtration with Sephadex G‐50. The final preparation obtained gave a single precipitin line with anti‐crude enterotoxin D serum and no precipitation with anti‐enterotoxin A, B or C when it was tested by Ouchterlony's technique. It was capable of producing 100% emesis in monkeys at about 1.25 μg of protein per kg body weight. Antiserum prepared by injecting rabbits with the purified preparation gave a single precipitin line with concentrated crude toxin, and it also gave a single arc when tested in immunoelectrophoresis with concentrated crude toxin. A type‐specific neutralization effect in monkeys was obtained. Thus, enterotoxin D has been identified with a purified preparation.