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Polycistronic Translation of Plant Viral Ribonucleic Acid
Author(s) -
Kiho Yukio
Publication year - 1972
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1972.tb00658.x
Subject(s) - tobacco mosaic virus , polysome , rna , translation (biology) , messenger rna , biology , virology , protein biosynthesis , ribosome , virus , microbiology and biotechnology , biochemistry , gene
Polysomes specific for tobacco mosaic virus (TMV) infection were analyzed. Two to three days after inoculation of tobacco leaves with TMV, polysomes of the large size, synthesizing TMV‐antigenic protein were observed. Molecular weight of TMV‐antigenic protein found under these conditions ranged from 2×10 4 to 6–8×10 4 daltons which might represent coat protein subunit and its trimer. Studies on the structure of polysomes bearing infecting RNA as messenger suggested that cistrons near the 3′‐end of TMV‐RNA was translated at first immediately after infection. These results favored the concept of a polycistronic translation of plant viral RNA, in contrast to a monocistronic translation observed in the mammalian system.