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Formation of Multiple Drug Resistance Factors by Recombination between Transfer Factor and Resistance Determinants
Author(s) -
Kameda Mitsuo,
Harada Kenji,
Suzuki Mitsue,
Nakajima Takashi,
Mitsuhashi Susumu
Publication year - 1972
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1972.tb00650.x
Subject(s) - biology , escherichia coli , chloramphenicol , genetics , recombinant dna , drug resistance , tetracycline , multiple drug resistance , recombination , streptomycin , microbiology and biotechnology , virology , gene , antibiotics
Drug‐resistance( r ) determinants, r 21 ( tet ), r 1 ( cml ), and r 11 ( str.sul ), were obtained from R factors, and were capable of conferring resistance to tetracycline, chloramphenicol, and to both streptomycin and sulfanilamide, respectively. They were integrated into the chromosome of Escherichia coli K12 and nontransmissible by conjugation. As described previously, the recombinant T‐ tet factor was formed and conjugally transmissible when E. coli K12 r 21 ( tet ) + was infected with T 95 , one of the transfer factors. Similarly, the recombinants T‐ tet.cml and T‐ tet.str.sul factors were formed by the interaction between T‐ tet factor and resistance determinants r 1 ( cml ) and r 11 ( str.sul ), respectively. Subsequently, T‐ tet.cml.str.sul factor was formed by the recombination between T‐ tet.cml factor and r 11 ( str.sul ) determinant, and T‐ tet.str.sul.cml factor was produced by the infection of E. coli K12 r 1 ( cml ) + with T‐ tet.str.sul factor. These recombinants were conjugally transmissible and transduced by phage Pl as one unit. These results strongly suggested the origin of R factors which were capable of conferring multiple resistance and transmissible by conjugation. According to the segregational patterns of resistance determinants by transduction and by conjugal transmission, the genetic structure of T‐ tet.cml.str.sul factors was established as being circular and the linkage order of the determinants of these factors was described.