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Immune Response against Hamster Erythrocytes in the Low‐Responder Mouse Strains
Author(s) -
Nomoto Kikuo,
Mashiba Harukazu,
Takeya Kenji
Publication year - 1972
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1972.tb00626.x
Subject(s) - antibody , immune system , antigen , adjuvant , titer , hamster , antibody response , microbiology and biotechnology , chemistry , strain (injury) , antibody titer , ratón , inbred strain , subcutaneous injection , biology , immunology , medicine , endocrinology , biochemistry , gene
Antibody response against hamster red blood cells (H‐RBC) was examined in inbred strains of C57BL/6, AKR, C3H/He, DDD and SL mice, and outbred CF1 mice. 1) There were strain differences in antibody response after a primary intravenous injection of H‐RBC. DDD, SL and CF1 mice belonged to high‐responder strains, while C57BL/6, AKR and C3H/He to low‐responder strains. In the spleens of immunized CF1 and SL, 40 to 70 times as many plaque‐forming cells (PFC) as those in C57BL/6 mice were detected. The magnitudes of the response were: CF1 ≒ SL>DDD>>C3H/He ≑ AKR>C57BL/6. 2) 2‐mercaptoethanol resistant (MER) antibody was detected in neither low‐ nor high‐responders after a primary intravenous antigen‐injection. 3) After a secondary intravenous antigen‐injection, MER antibody was detected in all the SL mice, but only in 30 to 50% of AKR and C57BL/6 mice. 4) A subcutaneous injection of H‐RBC in Freund's complete adjuvant (FCA) did not elicit antibody production within 10 days. When mice pre‐sensitized 7 days in advance w T ith H‐RBC in FCA were intravenously injected with H‐RBC, enhanced antibody production of the primary type was observed in all the mouse strains. 5) In pre‐sensitized mice, the extent of the enhancement of antibody production was the highest in low‐responder C57BL/6 mice and the lowest in high‐responder SL and CF1 strains. Thus, there was no strain difference in antibody titers or the numbers of PFC after the booster.