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Delayed Type Hypersensitivity in Hamsters Infected with Mycoplasma pneumoniae as Revealed by Macrophage Migration Inhibition Test
Author(s) -
Arai Sumio,
Hinuma Yorio,
Matsumoto Keizo,
Nakamura Takashi
Publication year - 1971
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1971.tb00612.x
Subject(s) - macrophage , antigen , mycoplasma pneumoniae , delayed hypersensitivity , mycoplasma , biology , antibody , microbiology and biotechnology , exudate , immunology , hamster , immunity , immune system , in vitro , medicine , pneumonia , biochemistry , botany
A delayed type hypersensitivity was demonstrated in hamsters infected with a fresh isolate of Mycoplasma pneumoniae by the following two procedures of macrophage migration inhibition test. In the first procedure, inhibition of migration of peritoneal exudate cells from normal guinea pigs by the addition of splenic cells from infected hamsters in the presence of the mycoplasma antigen was examined. In the second procedure, migration of peritoneal exudate cells, composed of macrophages and lymphocytes, from infected hamsters was examined after the addition of the antigen. Virtually, both procedures gave a similar result, suggesting the presence of cell‐mediated immunity in the infected animals. In these infected hamsters, humoral antibody responses were also demonstrated at the same time.