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Responses of Calf Kidney Cells to Human Adenovirus Type 12
Author(s) -
Kimura Susumu
Publication year - 1971
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1971.tb00605.x
Subject(s) - chemistry , dna , complement fixation test , hela , antigen , dna synthesis , kidney , microbiology and biotechnology , cell , cell culture , biology , antibody , immunology , biochemistry , serology , genetics , endocrinology
ABSTRACT The process of infection of established calf kidney (CKT) cells with human adenovirus type 12 (Ad 12) was compared with that with type 5 (Ad 5). Ad 5 replicated in CKT cells but Ad 12 did not. In CKT cells infected with Ad 5, as much virion antigen was detected by complement‐fixation (CF) test as from HeLa cells, and the rate of DNA synthesis increased to about 7 times that in uninfected cells. In CKT cells infected with Ad 12, production of T‐antigen was detected by CF test, but neither production of virion antigen nor increase in DNA synthesis was observed. Confluent monolayers of CKT cells incorporated 3 H‐thymidine progressively into DNA after growth medium was replaced with maintenance medium. This increase in host cell DNA synthesis after changing media was inhibited by infection with Ad 12 or with ultraviolet‐irradiated Ad 12.