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Studies on the Antigenic Activities of Yeasts
Author(s) -
Suzuki Shigeo,
Hatsukaiwa Hisanori,
Sunayama Hiroyoshi,
Honda Naoko
Publication year - 1971
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1971.tb00601.x
Subject(s) - mannan , concanavalin a , candida albicans , serotype , corpus albicans , antigen , lectin , agar , microbiology and biotechnology , serology , chemistry , precipitin , antibody , biology , biochemistry , bacteria , polysaccharide , immunology , in vitro , genetics
Five antigenic mannans isolated from the cells of Candida albicans serotype A, C. albicans serotype B, C. tropicalis, C. stellatoidea and Saccharomyces cerevisiae were examined for their reactivities against concanavalin A, the size of the combining site has been estimated to be relatively small, up to 4 hexopyranosyl residues. In the quantitative precipitation reaction, all mannan‐concanavalin A systems afforded nearly the same amounts of nitrogen or mannan precipitated, and the ratios of precipitation‐inhibition with α1→2 linked manno‐oligosaccharides, from biose to tetraose, were also equal regardless of the structural differences of these mannans. Furthermore, in agar‐gel double diffusion analysis, all the systems gave a corresponding precipitation arc which completely fused with the adjacent ones. These behaviors of mannan‐concanavalin A systems resemble those of antigen‐antibody systems consisting of the same mannans and anti‐C. albicans serotype B serum. It also provided evidence that the previous interpretation on the lesser serologic specificity of this serum compared to that of anti‐C. albicans serotype A was due to the smaller size of combining sites for antibodies of the former than of the latter serum.