Premium
Erythrocytes of Japanese Quail for Hemagglutination by Rubella Virus
Author(s) -
Soda Kenji,
Matumoto Minoru,
Tabata Eizo
Publication year - 1971
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1971.tb00591.x
Subject(s) - quail , titer , hemagglutinin (influenza) , coturnix , coturnix coturnix , biology , hemagglutination , rubella , rubella virus , ovotransferrin , virus , newcastle disease , albumin , chemistry , virology , biochemistry , transferrin , endocrinology , measles , vaccination
Erythrocytes (RBC) of adult Japanese quail, Coturnix coturnix japonica , were found to be as sensitive as day‐old chick RBC for rubella virus hemagglutination (HA). Various factors involved in the HA were studied with quail RBC as well as with adult pigeon and day‐old chick RBC. Pigeon RBC, unlike chick and quail RBC, tended to show, without hemagglutinin, a pattern of sedimented RBC resembling the HA pattern by the virus at 4 C, to a lesser extent at room temperature, in 0.85% NaCl solution buffered with m/100 phosphate (PBS), and were prevented from doing so by addition of a small amount of bovine plasma albumin to the diluent. A small amount (about 10 −3 m ) of CaCL 2 in PBS gave higher HA titers. The HA titer was higher at 4 C than at room temperature and much reduced at 36 C with chick and quail RBC. With pigeon RBC, addition of bovine plasma albumin to the diluent tended to reduce HA titers. The HA titer was highest at pH 5.8 to 6.8, and was inversely proportional to the RBC concentration. Under the conditions established on the basis of these findings, chick and quail RBC gave similar HA titers, but pigeon RBC gave consistently higher titers. However, these types of RBC gave no significant difference in HA‐inhibiting antibody titer when 4 units of hemagglutinin as determined with the homologous RBC was used.