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Studies on the Persistent Infection with Measles Virus in HeLa Cells
Author(s) -
Minagawa Tomonori
Publication year - 1971
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1971.tb00588.x
Subject(s) - measles virus , hela , clone (java method) , virology , virus , measles , biology , antigen , cloning (programming) , hemagglutinin (influenza) , cell culture , viral culture , microbiology and biotechnology , immunology , gene , genetics , vaccination , computer science , programming language
HeLa/MV cells which have previously been found to contain measles antigen in more than 90% of cells were very similar to normal HeLa cells in their morphology and growth. Although almost all of HeLa/MV cells may be infected, only 10% of them released infectious virus particles. The hemadsorption test, however, showed that most of the infected cells produced by hemagglutinin. Two kinds of clones were obtained by cloning HeLa/MV cells in the presence of anti‐measles serum. One was the virus‐releasing clone and the other the uninfected clone which did not contain any measles antigen. The proportions of virus‐releasing clones to all clones varied between 20 to 54%, and did not show any increase even after recloning of the virus‐releasing clones. The susceptibility of the uninfected clones to the standard measles virus was not different from that of normal HeLa cells.