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Slime Production by Pseudomonas aeruginosa
Author(s) -
Goto Sachiko,
Enomoto Seiji,
Takahashi Yoshio,
Motomatsu Reizo
Publication year - 1971
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1971.tb00587.x
Subject(s) - agar , pseudomonas aeruginosa , incubation , microbiology and biotechnology , pseudomonas , yeast extract , pseudomonas fluorescens , agar plate , nutrient agar , yeast , yield (engineering) , biology , chemistry , bacteria , food science , biochemistry , fermentation , genetics , materials science , metallurgy
Adequate experimental conditions for slime production by Pseudomonas aeruginosa were investigated using a cellophane plate method. Definite slime production was observed on heart infusion agar, brain heart infusion agar, yeast extract agar and synthetic agar, but not on nutrient agar. The addition of phosphate to the nutrient agar above 0.05% caused visible slime formation. Incubation at 37 C resulted in a higher yield of slime than at 25 C. Longer incubation seemed more favorable for slime production, while the pH reaction of the test media did not effect the slime yield. All the test cultures of P. aeruginosa produced large amounts of slime by this procedure. Cultures of Pseudomonas fluorescens , other Pseudomonas spp. and certain vibrios also produced slime under these experimental conditions.