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Phosphorylated Inactivation of Aminoglycosidic Antibiotics by Pseudomonas aeruginosa
Author(s) -
Kobayashi Fujio,
Yamaguchi Masahito,
Mitsuhashi Susumu
Publication year - 1971
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1971.tb00578.x
Subject(s) - paromomycin , kanamycin , antibiotics , gentamicin , neomycin , pseudomonas aeruginosa , microbiology and biotechnology , alkaline phosphatase , dihydrostreptomycin , aminoglycoside , chemistry , streptomycin , biology , bacteria , biochemistry , enzyme , genetics
The studies were conducted on the inactivation of aminoglycosidic antibiotics including a new antibiotic, lividomycin, by a cell‐free fraction from a strain of Pseudomonas aeruginosa isolated from the urine of a patient with cystitis. The strain strongly inactivated No. 2230‐C (mannosyl paromomycin), paromomycin, kanamycin, aminodeoxykanamycin and neomycin but did not inactivate lividomycin A, dihydrostreptomycin and gentamicin. Inactivated‐No. 2230‐C, ‐paromomycin and ‐kanamycin were isolated and purified using column chromatography techniques. Inactivated antibiotics were found to be reactivated by the treatment with alkaline phosphatase. According to the reactivation by alkaline phosphatase and analytical data, it was concluded that the inactivated products were monophosphorylated derivatives of each antibiotic.