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Crystallization of Turnip Yellow Mosaic Virus in Wilted Leaves of Chinese Cabbage by Treatment at High Temperature
Author(s) -
Ushiyama Rokuo
Publication year - 1971
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1971.tb00577.x
Subject(s) - turnip yellow mosaic virus , cytoplasm , wilting , ribosome , crystallization , chloroplast , biology , virus , chemistry , botany , crystallography , rna , plant virus , biochemistry , virology , organic chemistry , gene
Electron microscopic examination of Chinese cabbage leaves systemically infected with turnip yellow mosaic virus (TYMV) showed that numerous particles assumed to be virus were present in the cytoplasmic spaces between clumped chloroplasts. In the general ground cytoplasm TYMV particles were undistinguishable from the ribosomes. When whole leaves, detached from intact plants showing mosaic, were heated at 60 C for 5 min and kept at room temperature (about 20 C) for 18 hr, particles located in the spaces between chloroplasts and general cytoplasm were apparently crystallized. No such crystalline arrays were seen in healthy cells. The interparticle distance in the crystals formed in wilted tissue was 270–300 A. There was no evidence that cytoplasmic ribosomes have a tendency to crystallize under such conditions. It is suggested that TYMV particles are crystallized by the wilting procedure used.