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Morphological Changes in Pseudomonas aeruginosa Treated with Rod‐Shaped Pyocin 28
Author(s) -
Ohnishi Yoshinari,
Takade Akemi,
Takeya Kenji
Publication year - 1971
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1971.tb00571.x
Subject(s) - bacteriology , colicin , pseudomonas aeruginosa , microbiology and biotechnology , library science , medicine , biology , escherichia coli , computer science , genetics , bacteria , gene
purified by ammonium sulfate fractionation and 3 cycles of differential centrifugation, was added to a log-phase culture (5.8•~ Time (min) Fig. 1. Kinetics of adsorption of pyocin 28 to Pseudomonas aeruginosa P29 at 37C. Cells grown in nutrient broth to a concentration of 5.8•~108cells/ml were treated with various concentrations of pyocin 28 (closed circles), diluted at the indicated times to stop adsorption, and plated for survivors. To controls were added dilution fluid containing 10 mnr Tris-HCl buffer, pH7.5 (open circles).