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Inhibition of Phage Growth by an Antibiotic Rugulosin Isolated from Myrothecium verucaria
Author(s) -
Nakamura Shinichi,
Nii Fusako,
Shimizu Masanao,
Watanabe Itaru
Publication year - 1971
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1971.tb00560.x
Subject(s) - microbiology and biotechnology , antibiotics , multiplicity of infection , bacteria , biology , dna , rna , virology , virus , genetics , gene
It was found that (−)rugulosin, an antibiotic isolated from Myrothecium verucaria , had a potent anti‐phage effect on RNA phages (MS2, GA and Qβ) and DNA phages (δA and T4). The effect was almost independent of the host bacterial strains used. By a detailed investigation using MS2, it was revealed that the antibiotic did not affect the free phage or host bacterium alone but inhibited phage multiplication, and the degree of the inhibition depended on the multiplicity of infection. The inhibition was not mainly due to a drop in the burst size but rather was due to a decrease of the phage‐producing cells during the early stages of phage infection and replication.