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RNA‐Induced Interference with Animal Virus Infection
Author(s) -
Matsuyama Masako,
Kawade Yoshimi,
Fukada Tetsuo
Publication year - 1970
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1970.tb00528.x
Subject(s) - rna , cycloheximide , biology , protein biosynthesis , rna interference , embryo , virus , microbiology and biotechnology , rna silencing , virology , biochemistry , gene
Some RNAs, including both single‐ and double‐stranded RNAs, when incubated with chick embryo cell culture induce cellular resistance against viruses. Evidence was now obtained indicating that the induction of cellular resistance by RNA depends on the cellular metabolic activity, especially on the synthesis of cellular RNA and protein. Thus, inhibitors of RNA and protein synthesis, actinomycin D and cycloheximide, were found to inhibit the development of an antiviral state when added before, or during the relatively early period of, incubation of the cells with RNA. In the course of induction of cellular resistance, three stages may be distinguished, the priming stage, the developing stage, and the established resistant stage.