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Determination of Pseudomonas aeruginosa by Biochemical Test Methods
Author(s) -
Arai Taketoshi,
Enomoto Seiji,
Goto Sachiko
Publication year - 1970
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1970.tb00526.x
Subject(s) - incubation , microbiology and biotechnology , pseudomonas aeruginosa , citrobacter , nutrient agar , agar , pseudomonas fluorescens , aeromonas , vibrio parahaemolyticus , strain (injury) , chemistry , enterobacter , bacteria , biology , tryptic soy broth , incubation period , serratia marcescens , escherichia coli , biochemistry , genetics , anatomy , gene
ABSTRACT A new synthetic medium consisting of 0.2% KH 2 PO 4 , 0.01% MgSO 4 ·7H 2 O, 0.5% NaCl, 0.05% (NH 4 ) 2 SO 4 and 1.0% Tween 80 was devised by the authors as a means of differentiating between Pseudomonas aeruginosa and related species of gram‐negative rods. One loopful of an overnight nutrient agar culture of a test strain was inoculated into 1 ml of this media, and incubated at 37 C for 24 hr. All of the 38 test strains of P. aeruginosa showed visible growth in this media, being capable of utilizing Tween 80 as a sole carbon source, whereas strains of Aeromonas, Vibrio parahaemolyticus, Achromobacter sp., Proteus sp., Escherichia coli and Citrobacter sp. could not grow within a 24 hr incubation period, although longer incubation yielded a visible growth with some strains. A strain of P. fluorescens did not show any visible growth at 37 C, but did show a moderate growth after a 48 hr incubation at 18 C. Strains of Klebsiella and Enterobacter showed only slight growth after incubation at 37 C for 24 hr.