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Genetic Recombination in Klebsiella pneumoniae An Approach to Genetic Linkage Mapping
Author(s) -
Matsumoto Hideki,
Tazaki Tadakatu
Publication year - 1970
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1970.tb00500.x
Subject(s) - klebsiella pneumoniae , genetics , genetic linkage , biology , recombination , genetic recombination , escherichia coli , linkage (software) , chromosome , strain (injury) , klebsiella , salmonella , mutation , gene , enterobacteriaceae , microbiology and biotechnology , bacteria , anatomy
Genetic recombination was observed between two different strains of Klebsiella pneumoniae , which is a non‐motile and encapsulated bacterium belonging to the family Enterobacteriaceae and has about 55% of its DNA content as GC. The mode of recombination seemed to be similar to that of the F‐factor mediated conjugation in Escherichia coli . One strain acted as the donor and the other as the recipient, and a relatively large fragment of the donor's chromosome was transferred unilaterally and unidirectionally by cell to cell contact. No genetic factor which is associated with the recombination has been identified. The genetic linkage map of K. pneumoniae was analyzed various mutants derived from the two strains. It was found that the 28 markers so far investigated were arranged linearly in a single linkage group, and that the genetic linkage map of K. pneumoniae , like that of E. coli , could be considered circular. The proposed genetic linkage map of K. pneumoniae was quite similar to that of E. coli or Salmonella typhimurium . The close similarities in this map among the three species suggest a possibility that K. pneumoniae may have differentiated from an ancestor common all three species.