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The Structure of Tobacco Mosaic Virus from Base Analogue Treated Tobacco Leaves
Author(s) -
Nomura Akira,
Taniguchi Takeshi,
Hirai Tokuzo
Publication year - 1969
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1969.tb00453.x
Subject(s) - tobacco mosaic virus , chemistry , thiouracil , ion chromatography , biochemistry , chromatography , virology , virus , biology , thyroid , endocrinology
Structure of TMV containing the base analogues such as 2‐thiouracil, 8‐azaguanine, and 5‐fluorouracil was studied. TMV containing these analogues had the same anion‐exchange chromatographic properties as had normal TMV, and 2‐thiouracil was incorporated into TMV particles, probably into TMV‐RNA. Cation‐exchange chromatographic properties of TMV containing these analogues had no significant difference as compared with those of normal TMV. TMV containing 2‐thiouracil was more stable to alkali degradation than normal TMV. Alkali‐degraded TMV containing 2‐thiouracil and normal TMV was characterized by chromatography. These results suggest that not surface structure but internal structure of TMV containing 2‐thiouracil was different from that of normal TMV.