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Studies on the Antigenic Activities of Yeasts
Author(s) -
Suzuki Shigeo,
Sunayama Hiroyoshi
Publication year - 1969
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1969.tb00439.x
Subject(s) - mannan , chemistry , candida albicans , polysaccharide , hydrolysis , antigen , yeast , biochemistry , corpus albicans , paper chromatography , acid hydrolysis , column chromatography , chromatography , microbiology and biotechnology , biology , genetics
The antigenic mannan of Candida albicans was degraded by acid‐hydrolysis and the resultant oligosaecharides were fractionated by a carbon‐Celite and a subsequent cellulose‐powder chromatography to yield four oligosaecharides, pentaose, hexaose, heptaose and octaose, which involved 2,6‐di‐0‐ and 6‐0‐substituted mannopyranosyl residues as the common. feature. These oligosaccharides showed lower precipitation‐inhibition activity than that of the hexaose of acetolysate, the strongest inhibitor among the oligosaccharides described in the preceding study. The order of inhibitory powers of oligosaccharides was as follows: hexaose of acetolysate>heptaose>pentaosez=octaose>hexaose. The μmoles requiring for 50%‐inhibition were 0.025, 0.15, 0.20, 0.20 and 0.50 respectively. The results clearly indicate that the determinant groups of the mannan of C. albicans employed this study are the hexaose moieties which constitute the branching parts of polysaccharide.