Premium
Transfer Agent of Immunity
Author(s) -
Kawakami Masaya,
Kitamura Kouichi,
Mikami Hirokazu,
Mitsuhashi Susumu
Publication year - 1969
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1969.tb00431.x
Subject(s) - puromycin , spleen , cycloheximide , rna , antibody , in vitro , immune system , deoxyribonuclease , antigen , microbiology and biotechnology , proteolytic enzymes , biology , rosette (schizont appearance) , incubation , immunity , ribonuclease , chemistry , enzyme , protein biosynthesis , biochemistry , immunology , gene
Using erythrocytes as antigen particles, number of antibody‐forming cells was enumerated by immunocytoadehesion technique, in which formation of rosette was shown to be inhibited by anti‐mouse immunoglobulin sera. This number increased in vitro after treatment of spleen cells of mice for 60 min with RNA fraction extracted from spleen of mice immunized with erythrocytes used in the enumeration, and incubation of cells for 12 hr at 37 C. Response of cells treated with immune RNA fraction was immunologically specific and was inhibited by puromycin or cycloheximide. The activity of immune RNA capable of converting nonimmune cells to antibody‐forming cells was shown to be sensitive to ribonucleases but resistant to deoxyribonuclease and proteolytic enzyme.