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Immunochemical Studies on Bacterial Blood Group Substances
Author(s) -
Kochibe Naohisa,
Iseki Shoei
Publication year - 1968
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1968.tb00413.x
Subject(s) - fucose , escherichia coli , chemistry , enzyme , oligosaccharide , hydrolysis , biochemistry , galactose , gene
The terminal α‐galactosyl linkage was hydrolyzed by an enzyme preparation from either Clostridium maebashi or coffee beans. Reduction of blood group B activity of Salmonella milwaukee and Escherichia coli O 86 lipopolysaccharides and of some oligosaccharides occurred consequently. The degraded lipopolysaccharides showed significant blood group O (H) activity indicating the presence of terminal α‐fucosyl residues in them and the same activity was demonstrated with oligosaccharide A‐3 after the enzyme treatment. α‐Fucosidase derived from Bacillus fulminans caused liberation of fucose from the α‐galactosidasc‐treated, materials and abolishment of O (H) activity. The results of quantitative analysis, borohydride reduction, Morgan‐Elson reaction and treatments with several kinds of enzyme preparations on a series of oligosaccharides indicated that the structure of O‐somatic side chain of E. coli O 88 is probably;α ‐ D ‐ Gal ‐ ( 1 → 3 ) ‐ β ‐ D ‐ Gal ‐ D ‐ GalNAc ‐ ( 1 → 3 ) ‐ D ‐ GalNAc ‐ ( 1 → 4 ) ‐ Fuc ‐↑ 1 2α ‐ L ‐ Fuc(↑ β ‐ D ‐ G1c)It was evident that there is a similarity in the terminal structure of lipopolysaccharides of E. coli O 86 , S. milwaukee and human B blood group substance.