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Multiplication of Tobacco Mosaic Virus in Suspension Culture of Tobacco Cells
Author(s) -
Motoyoshi Fusao,
Oshima Nobuyuki
Publication year - 1968
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1968.tb00401.x
Subject(s) - tobacco mosaic virus , infectivity , in vitro , inoculation , callus , biology , nicotiana tabacum , virology , virus , suspension culture , pith , cell culture , microbiology and biotechnology , chemistry , botany , horticulture , biochemistry , genetics , gene
The present study was attempted to establish an in vitro system for TMV infection and multiplication. A friable cell line Bf1 was isolated from an original callus line which which obtained from a pith of Nicotiana tabacum . Cells of the line were stirred with ordinary TMV by means of a grass rod to be mechanically infected, suspended in the liquid medium, and incubated on a reciprocal shaker. Infectivity of TMV in the cells was determined by the local lesion assay with the leaves of N. glutinosa . It was increased in the incubated cells, reflecting that TMV was able to multiply in the in vitro system. An exponential increase of infectivity was observed during 2 and 5 days after inoculation.