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A New Cell Line of Calf Kidney and Its Susceptibility to Viruses
Author(s) -
Kimura Susumu,
Fukui Kohmei,
Yoshida Nagayuki,
Matsubara Yoshikatsu
Publication year - 1968
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1968.tb00398.x
Subject(s) - cell culture , echovirus , fibroblast , virology , poliovirus , biology , enterovirus , in vitro , hela , cell , embryo , virus , kidney , microbiology and biotechnology , genetics
A new cell line (CKT) derived from a male calf kidney obtained in Tokushima, Japan has been established and cultivated serially over a period of 3 years. Morphologically, this line had a fibroblast‐like appearance during early cell passages, but became an epithelial‐cell form by the 20th passage and subsequently retained this form without significant morphological change during further passage in vitro . CKT cells had no susceptibility to poliovirus types 1, 2 and 3 or to echovirus type 4, but low susceptibility to coxsackie viruses B1 and B5. The cells showed a susceptibility similar to that of HeLa cells, but lower than that of human embryo kidney cells, when challenged with adenoviruses.