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Immunological Specificity of Soluble Polysaccharides of Candida Albicans
Author(s) -
Fukazawa Yoshimura,
Elinov Nikolay P.,
Shinoda Takako,
Tsuchiya Takeshi
Publication year - 1968
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1968.tb00397.x
Subject(s) - polysaccharide , candida albicans , sephadex , chemistry , immunogenicity , chromatography , agglutination (biology) , biochemistry , microbiology and biotechnology , antibody , biology , enzyme , immunology
Specificity and immunogenicity of soluble polysaccharides of Candida albicans which were extracted with β‐naphthol, boiling water for 1 hr, and with β‐naphthol from cell residue were studied by agglutination and agglutinin absorption method. Soluble polysaccharides produced sufficient agglutinins of IgG nature. A high degree of specificity of soluble polysaccharides was proved, as specific antigen 7 of C. albicans existed in the soluble fractions. Studies on the nature of macromolecules of soluble polysaccharides performed by column chromatography on Sephadex G‐200 and DEAE‐Sephadex, indicated that molecular sizes and yields of neutral and acidic polysaccharides may vary according to methodology used. Soluble polysaccharide extracted with boiling water had a larger molecular size than that obtained with β‐naphthol. Present data on specificity of soluble polysaccharides as well as previous observations on specificity of IgG antibody suggest that IgG produced by immunization with soluble polysaccharide contains specific antibody factor capable of differentiating C. albicans from C. tropicalis .