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Transfer Agent of Immunity
Author(s) -
Mitsuhashi Susumu,
Kurashige Satonori,
Kawakami Masaya,
Nojima Tokukichi
Publication year - 1968
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1968.tb00394.x
Subject(s) - immune system , ribonuclease , flagellum , microbiology and biotechnology , exudate , antibody , antigen , salmonella , deoxyribonuclease , biology , rna , immunity , peritoneal cavity , virology , bacteria , immunology , enzyme , biochemistry , anatomy , genetics , botany , gene
Detection of the cells which contain antibody was accomplished by a method of immune adherence of human erythrocytes to a single cell, termed SCIA (single cell immune adherence) reaction. Peritoneal exudate cells were collected from mice immunized with flagella of either Salmonella enteritidis or S. tennessee . Serologically specific antibody was detectable in some of the peritoneal exudate cells of such mice. An immune ribonucleic acid (RNA) was extracted from the peritoneal exudate cells of mice immunized with salmonella flagella. When mice were injected intraperitoneally with this preparation, serologically specific antibody was found in some of their peritoneal exudate cells by the SCIA method. This preparation was inactivated by treatment with ribonuclease, but was resistant to proteinases, deoxyribonuclease and anti‐flagella antibody, suggesting that this agent is of RNA nature and does not contain antigen or fragment thereof.