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Further Studies on the Effect of 5‐Iododeoxyuridine on Polyoma Virus Multiplication in Mouse Cells
Author(s) -
Kimura Genki,
Mori Ryoichi,
Amako Kazunobu
Publication year - 1966
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1966.tb00310.x
Subject(s) - idoxuridine , virology , virus , embryo , biology , infectivity , cytopathic effect , tissue culture , complement fixation test , microbiology and biotechnology , kidney , cell culture , antibody , chemistry , in vitro , serology , immunology , biochemistry , genetics , endocrinology
5‐Iododeoxyuridine (IUDR) inhibited production of infectious polyoma virus in mouse embryo cells and mouse kidney cells in culture. Deoxythymidine reversed its effect. IUDR did not inactivate infectivity of free virus particles. IUDR did not prevent adsorption and penetration of polyoma virus to cells. The events sensitive to IUDR treatment occurred at around 20 hours after infection. The cytopathic effects of polyoma virus, including emergence of DNA containing‐inclusions in the nucleus, were observable in infected cells in which viral replication was completely arrested by IUDR. It was shown by fluorescent antibody technique in infected mouse embryo cells and by complement fixation test in infected mouse kidney cells that IUDR inhibited completely the synthesis of viral antigen. No virus‐like particles were demonstrated in the IUDR‐treated infected‐mouse kidney cells by electron microscope examinations.