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Behavior of Some Episomal Elements in a Recombination‐Deficient Mutant of Escherichia coli
Author(s) -
Takano Toshiya
Publication year - 1966
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1966.tb00309.x
Subject(s) - transduction (biophysics) , mutant , escherichia coli , biology , bacteria , recombination , plasmid , microbiology and biotechnology , bacteriophage , gene , lysogenic cycle , mutation , genetics , biochemistry
Conjugal transfer and autonomous replication of some episomes occurred normally in a recombination‐deficient (Rec – ) mutant of Escherichia coli K‐12. Transduction with phage Plbt of an R factor also occurred normally in this Rec – mutant, but complete or abortive transduction with Plbt of chromosomal genes did not occur. In contrast, transduction of galactose genes by phage λ dg occurred in the Rec – bacteria as frequently as in the Rec + strain. It was shown that phage Plbt does not grow at all on the Rec–bacteria. Recombination between two different R factors, two mutants of phage λ and two mutants of phage T4 occurred normally in the Rec – bacteria, but did not give a Rec + phenotype to the host bacteria. Colicinogenic factor I made the Rec – host bacteria more resistant to ultraviolet light but the colicinogenic strain was still infertile in the crosses with the Hfr srains of E. coli K‐12.