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Studies on Two Colonial Types of Leptospira icterohaemorrhagiae with Special Reference to the Bottle Culture Method
Author(s) -
Fujikura Takao
Publication year - 1966
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1966.tb00294.x
Subject(s) - virulence , leptospira , serotype , biology , bottle , microbiology and biotechnology , agglutination (biology) , agglutinin , direct agglutination test , leptospirosis , serology , virology , antigen , antibody , lectin , immunology , mechanical engineering , biochemistry , gene , engineering
ABSTRACT A bottle culture method is described which favours the colony formation of Leptospira icterohaemorrhagiae . The Kitakata strain formed two morphological types of colonies when grown in rubber‐stoppered 200 ml‐square bottle cultures. One was compact and small (C type), while the other diffuse and large (D type). The C and D type sub‐strains showed the following differences: The C type leptospirae were longer in body length, but less virulent for suckling mice, young hamsters, guinea pigs, or young rabbits. The C type leptospirae lost 90% of the colony forming units (CFU) after being heated at 45 C for 20 minutes. On the other hand, the D type leptospirae were shorter in body length, virulent in those animals examined and resistant to heating. No serological differences were observed between these two types of sub‐strains by agglutination test and in agglutinin absorption test.