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Studies on Habu Snake Venom
Author(s) -
Kurashige Satonori,
Hara Yoshihiko,
Kawakami Masaya,
Mitsuhashi Susumu
Publication year - 1966
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1966.tb00288.x
Subject(s) - snake venom , phospholipase , venom , chemistry , ammonium sulfate , chromatography , phospholipase a , fractionation , phospholipase a2 , ammonium , heat stability , homogeneous , biochemistry , enzyme , materials science , organic chemistry , thermodynamics , composite material , physics
It has been found that there are two factors responsible for myolysis in Habu snake venom; heat‐labile and heat‐stable myolytic factors. As reported previously, the former is a proteinase contained in Habu venom. In this article purification and chemical properties of the heat‐stable myolytic factor are presented. Using the method of fractionation with ammonium sulfate and with column chromatography, a heat‐stable myolytic factor was obtained ultracentrifugally in a homogeneous state. This factor caused severe myolysis with the aid of both Mg ++ ion and phospholipase A. It has been found that heat‐stable myolytic factor has no activities such as those of proteinase, esterase or ribonuclease. The relationship between the heat‐stable myolytic factor and phospholipase A in myolytic activity is discussed.