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THE DETECTION OF ANTIBODIES AGAINST ATYPICAL ACID‐FAST BACILLI IN THE SERUM OF TUBERCULOUS PATIENTS BY THE OUCHTERLONY METHOD
Author(s) -
Sushida Kiyo,
Hirano Norimasa
Publication year - 1962
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1962.tb00228.x
Subject(s) - bacteriology , ouchterlony double immunodiffusion , medicine , medical science , library science , family medicine , antibody , biology , computer science , immunology , medical education , genetics , antiserum , bacteria
Previously, Hirano and Sushida(1) investigated the antigenic relationship between mammalian tubercle bacilli and atypical mycobacteria, and they found a serologically homogeneous group among 12 strains which had been isolated at our hospital. Atypical mycobacteria are infrequently isolated from sputa of tuberculous patients, and many of the atypical mycobacterial strains tested by the authors(2,3) showed that tuberculous changes are produced by intratesticular inoculation of rabbits. Parlett, Youmans, Rehr and Lester(4) tested the sera obtained from 53 patients for the presence of circulating antibody by means of tube double-diffusion precipitation method, and they found that all but possessed antibodies. The antigen used in the experiment was the concentrated culture filtrate of the H37Rv culture of Mycobacterium tuberculosis var. hominis. Thuston and Steenken, JR.(5) employed unheated, concentrated culture filtrates of the H37Rv or H37Ra strains of tubercle bacilli as antigen, and they tested the sera of 276 tuberculous subjects by the double diffusion gel precipitation technique. The results indicated that 59 of the 73 serum specimens (81 per cent) from cases of active far advanced tuberculosis and 79 of the 120 specimens (66