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THE CHROMATOGRAPHIC PURIFICATION OF BACTERIOPHAGE AND ENDOTOXIN OF PSEUDOMONAS AERUGINOSA
Author(s) -
Homma J. Yuzuru,
Hamamura Norikatsu,
Ashizawa Yoshio
Publication year - 1961
Publication title -
japanese journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0021-5139
DOI - 10.1111/j.1348-0421.1961.tb00195.x
Subject(s) - library science , citation , pseudomonas aeruginosa , computer science , biology , bacteria , genetics
Studies(1) on the endotoxin of Pseudomonas aeruginosa derived from synthetic fluid culture was shown to be homogeneous by electrophoresis and ultracentrifugation analyses. The chemical characterization of the endotoxin showed it to be a lipopolysaccharide-protein complex with a molecular weight of one million. On the other hand, Pseudomonas aeruginosa P1-III strain, which has been used for the studies of endotoxin, was found to be lysogenic and produced 105 phage particles per ml in the synthetic medium(2). The separation of phage from the endotoxin and its purification were carried out in the following manner. The endotoxin was successfully isolated by means of carboxylic acid ion exchange resin column chromatography. Phages were separated, from antigenic substances by subjecting the phage solution to column chromatography using basic ion exchange resins. For the chromatographic purification of proteins such as lysozyme(3), ribonuclease(4), bacteriophage(5), and viruses(6,7,8) carboxylic acid ion exchange resin has been used previously.