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Anti‐CXCR3 staining is useful for detecting human cutaneous and mucosal mast cells
Author(s) -
FUKIWAKE Noriko,
MOROI Yoichi,
IMAFUKU Shinichi,
MASUDA Teiichi,
KOKUBA Hisashi,
FURUE Masutaka,
URABE Kazunori
Publication year - 2006
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.2006.00077.x
Subject(s) - tryptase , staining , chymase , pathology , immunohistochemistry , cxcr3 , immunofluorescence , human skin , chemistry , mast cell , antibody , medicine , biology , immunology , inflammation , chemokine , chemokine receptor , genetics
Human synovial mast cells (MC) can be immunolabelled with antihuman CXCR3 antibody (Ab) (clone 49801). We have investigated whether cutaneous and mucosal MC are stained with anti‐CXCR3 Ab in paraffin‐embedded sections. Immunohistochemical staining and immunofluorescence double staining assays were performed with anti‐CXCR3, anti‐tryptase, and anti‐chymase Ab using normal skin, psoriatic skin lesions, and normal colon. When compared with tryptase and chymase staining, 100% of the cutaneous and 98% of the mucosal MC were positive for CXCR3. Anti‐CXCR3 staining is a useful marker for human cutaneous and mucosal MC in paraffin‐embedded sections.