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Pharmacological Profiles of High‐Concentration (20 μg/g) Tacalcitol Ointment: Effects on Cutaneous Inflammation, Epidermal Proliferation, and Differentiation in Mice
Author(s) -
Sato Hiroaki,
Ogino Yasuhiro,
Takagi Hideko,
Hata Junko,
Asano Satoshi,
Ohta Tomohiro,
Komoriya Keiji
Publication year - 2003
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.2003.tb00425.x
Subject(s) - medicine , hairless , inflammation , pharmacology , chemistry , immunology , biochemistry
This study focused on the effects of tacalcitol (1,24(R)(OH) 2 D 3 , TV‐02) ointment (20 μg/g) on cutaneous inflammation, epidermal proliferation, and differentiation and compared them with tacalcitol ointment (2 μg/g) and other anti‐psoriatic ointments using hairless mice. Tacalcitol ointment (0, 2 and 20 μg/g) significantly inhibited 12‐ O ‐tetradecanoylphorbol 13‐acetate (TPA)‐induced cutaneous inflammation, histopathologically. The effect of tacalcitol ointment (20 μg/g) on cutaneous inflammation was much stronger than that of tacalcitol ointment (0, 2 μg/g), and as effective as calcipotriol ointment (50 μg/g) or betamethasone valerate ointment (1.2 mg/g). Tacalcitol ointment (20 μg/g) also significantly inhibited TPA‐induced myeloperoxidase (MPO) activity, as effectively as calcipotriol ointment (50 μg/g) or betamethasone valerate ointment (1.2 mg/g). The effect of tacalcitol ointment on epidermal proliferation [ornithine decarboxylase (ODC) activity] and differentiation [transglutaminase (TGase) activity] was dose‐dependent from 0 μg/g to 20 μg/g. The effect of tacalcitol ointments on epidermal proliferation was significant at the doses of 2 μg/g and 20 μg/g, and that on epidermal differentiation was significant at the doses of 0.2 μg/g or more. The effect of tacalcitol ointment (20 μg/g) on epidermal differentiation was significantly stronger than tacalcitol ointment (2 μg/g). In this study, tacalcitol ointment (20 μg/g) was found to have a marked effect on cutaneous inflammation and improved effect on epidermal differentiation, although tacalcitol ointment (2 μg/g) also had significant effects on epidermal proliferation and differentiation. These findings support the clinical effectiveness of tacalcitol ointment (20 μg/g) against psoriasis.