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A Case of Bullous Pemphigoid Successfully Treated by Plasmapheresis: Assessment of the Change in Titers of Circulating Antibodies by Immunoblotting and Enzyme‐Linked Immunosorbent Assay
Author(s) -
Lee Jee Bum,
Fumimori Takeaki,
Kurose Koichiro,
Mori Osamu,
Hashimoto Takashi
Publication year - 2003
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.2003.tb00395.x
Subject(s) - bullous pemphigoid , autoantibody , plasmapheresis , titer , recombinant dna , antibody , medicine , immunology , antigen , pemphigoid , chemistry , biochemistry , gene
We report a case of bullous pemphigoid successfully treated with double filtration plasmapheresis. The changes in titers of circulating autoantibodies were assessed by immunoblotting and enzyme‐linked immunosorbent assay (ELISA) using a recombinant protein of the non‐collagenous 16a (NC16a) domain of the 180 kDa bullous pemphigoid antigen (BP180). The ELISA was shown to be more sensitive in detecting disease‐specific antoantibodies in the bullous pemphigoid sera. The reduction of titers of circulating autoantibodies in the sera correlated well with the decrease in the disease activity in both the first and second rounds of plasmapheresis treatment in this case.