Premium
Detection of Species of the Subgenus Leishmania Parasites Using Polymerase Chain Reaction and Southern Blotting
Author(s) -
Uezato Hiroshi,
Takei Kimiko,
Maruno Motoyoshi,
Khaskhely Noor Mohammad,
aka Shigeo,
Oshiro Minoru,
Kariya Kenichi,
Katakura Ken,
Mimori Tatsuyuki,
Gomez L. Eduardo A.,
Furuya Masato,
Shamsuzzaman S.M.,
Hashiguchi Yoshihisa
Publication year - 2001
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.2001.tb00014.x
Subject(s) - subgenus , polymerase chain reaction , biology , leishmania , primer (cosmetics) , southern blot , microbiology and biotechnology , virology , parasite hosting , dna , genetics , taxonomy (biology) , zoology , gene , chemistry , organic chemistry , world wide web , computer science
In this study, an attempt was made to identify different Leishmania species by polymerase chain reaction (PCR). Fourteen Leishmania strains from stock were tested by PCR and Southern blotting. A pair of primers were employed that anneal to the kinetoplast DNA sequence conserved among subgenus Leishmania. Of the 14 Leishmania strains used in this study, six showed strong bands of approximately 170 bp, and all the positive strains belonged to the species of the subgenus Leishmania viz., Leishmania (Leishmania) garnhami, L. (L.) amazonensis, L. (L.) pifanoi, L. (L.) mexicana, L. (L.) chagasi , and L. (L.) major. All the species belonging to the subgenus Viannia used in this study were negative by PCR. These results suggest that the primer pair may be useful for identification of the species belonging to the subgenus Leishmania of the New World as well as to distinguish subgenus Leishmania from subgenus Viannia.