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Analysis of T‐Cell Antigen Receptor γ Chain Gene Rearrangement by Polymerase Chain Reaction in Combination with Denaturing Gradient Gel Electrophoresis in the Differential Diagnosis of Cutaneous T‐Lymphoproliferative Diseases
Author(s) -
Nagasawa Tomohiko,
Nakatsuka Shinichi,
Miwa Hideaki,
Kanno Hiroyuki,
Itami Satoshi,
Yoshikawa Kunihiko,
Aozasa Katsuyuki
Publication year - 2000
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.2000.tb02158.x
Subject(s) - mycosis fungoides , gene rearrangement , t cell receptor , pathology , biopsy , polymerase chain reaction , skin biopsy , acute lymphocytic leukemia , lymphoproliferative disorders , cd3 , leukemia , biology , medicine , t cell , antigen , lymphoma , immunology , gene , immune system , lymphoblastic leukemia , biochemistry , cd8
A polymerase chain reaction (PCR)‐based strategy has been developed for analysis of clonal rearrangement of the T‐cell receptor γ gene (TCRγ) and was shown to be useful for detection of clonal T‐cell populations. In this study, we performed PCR combined with denaturing gradient gel electrophoresis (DGGE) on fresh frozen biopsy samples from 16 patients with cutaneous T‐lymphproliferative diseases in whom a definite diagnosis was difficult to make on morphological and immunohistochemical grounds alone. Ages of the patients at biopsy ranged from 28 to 81 (median 62) years, and the subjects consisted of 8 men and 8 women. They presented with erythema on the extremities in 5 cases, trunk in 7, buttock in 2, and papules on the trunk and face in one case each. Clonal rearrangement of TCRγ was observed in 3 of 16 cases. Clinical diagnoses of these three cases were mycosis fungoides, cutaneous invasion of adult T‐cell leukemia (ATL), and large granular lymphocytic leukemia (LGL) of T‐cell type, respectively, but they were histologically difficult to differentiate from reactive cutaneous T‐cell proliferation. The skin lesions of the LGL case worsened, and this patient died two years after biopsy. Another patient with suspected mycosis fungoides in the plaque stage died due to dissemination of tumors 22 months after biopsy. The remaining one patient with ATL survived with cutaneous lesions for over four years. Clonality was not demonstrated in the remaining 13 cases, and their clinical courses were favorable. These findings showed that demonstration of clonal TCRγ gene rearrangement using the PCR‐DGGE method is very helpful for diagnosis of cutaneous T‐cell neoplasms.